CAS: 77591-33-4 · MW: 4963.55 Da · Purity: ≥99% by reverse-phase HPLC · Format: 10 mg powder · Identity: ESI-MS confirmed
This is the 10 mg vial of TB-500, a synthetic peptide based on the active region of thymosin beta-4. The larger vial suits research that works through more material. It is the same fragment-based peptide as the 5 mg size, studied for the actin and tissue-repair biology that thymosin beta-4 is known for.
Structure & Identity
TB-500 reproduces the full 43-residue sequence of thymosin beta-4, an acetylated peptide (Ac-SDKPTSFDKSKLKKTETQEKNPLPSKETIEQEKQAGES) with a molecular weight of 4963.55 Da and CAS number 77591-33-4. The N-terminal acetyl group is a natural modification that comes from how the body produces the peptide. Within that chain sits the actin-binding LKKTETQ region, the part responsible for the actin-sequestering behavior that defines thymosin beta-4 in research, and the reason the peptide is studied for cell migration and tissue repair.
Research Background
Research on TB-500 draws on thymosin beta-4’s role in cell movement and tissue regeneration.
- Cell migration. As an actin-sequestering protein, thymosin beta-4 supports the cell migration that underpins tissue repair, documented in foundational research [1].
- Multifunctional regeneration. It is described in reviews as a regenerative peptide acting across several repair processes [2].
- Fragment activity. The mapping of thymosin beta-4’s activity onto short peptide sequences supports the study of fragments like TB-500 [3].
These describe research areas, not approved uses, and the peptide is provided for laboratory study.
Specifications
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Attribute
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Value
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Chemical name
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TB-500 (thymosin beta-4 fragment, actin-binding region)
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Also Known As
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Thymosin Beta-4, Tβ4
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CAS number
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77591-33-4
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Molecular weight
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4963.55 g/mol
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Length
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43 amino acids (acetylated)
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Purity
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≥99% by reverse-phase HPLC
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Identity
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Confirmed by ESI mass spectrometry
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Physical form
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Lyophilized powder
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Appearance
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White to off-white solid
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Quantity
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10 mg per vial
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Storage
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-20°C, protected from light
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Solubility
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Soluble in bacteriostatic or sterile water
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Classification
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Research use only
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Storage & Handling
Keep the unopened vial at -20°C, away from light, until needed. Once dissolved, store the solution at 2-8°C and use within roughly two weeks. Let the vial reach room temperature before opening to limit condensation, and aliquot the reconstituted material so it is not frozen and thawed repeatedly.
Storage and Handling Guide
Quality Assurance
Every lot of TB-500 clears independent quality control before release. Manufacturing follows research-grade standards, and purity by reverse-phase HPLC and identity by mass spectrometry are confirmed by outside laboratories, including Janoshik Analytical and US-based labs. A batch-matched certificate of analysis accompanies the vial so the data traces to your material.
Quality & Testing
Frequently Asked Questions
What is the TB-500 10 mg used for in research?
It is a thymosin beta-4 fragment studied in tissue-repair and cell-migration research. TB-500 is a research-use-only material with no approved human use in this setting.
Is the 10 mg the same as the 5 mg TB-500?
Yes, apart from the amount of peptide in the vial. The 10 mg size suits research needing more working material.
Is TB-500 the full thymosin beta-4 protein?
No, it corresponds to the active fragment of thymosin beta-4, not the whole protein. Suppliers label this inconsistently, which is why the exact identity should be confirmed.
How does TB-500 relate to actin?
Thymosin beta-4 binds and sequesters actin, which helps regulate the cell movement involved in tissue repair. TB-500 is studied as a fragment carrying that activity.
Is TB-500 FDA approved?
No, it is not FDA approved. It featured in the 2026 FDA compounding review but was not approved. This listing is a research-use-only reference peptide, not for human or veterinary use.
Why are the CAS and molecular weight flagged “confirm”?
Because “TB-500” can mean the fragment or, per its listed CAS, the full protein. The precise identity should be verified against the actual material before publishing.
How should the 10 mg vial be handled?
Keep it frozen at -20°C and out of light. After reconstitution for research, refrigerate at 2-8°C, aliquot, and use within about two weeks.
How is quality verified?
Purity is confirmed at ≥99% by reverse-phase HPLC with mass-spec identity confirmation by an independent lab, and a certificate of analysis is included with each batch.
References
- Thymosin beta4: actin-sequestering protein moonlights to repair injured tissues. 2005. PMID: 16099219.
- Thymosin β4: a multi-functional regenerative peptide. 2012. PMID: 22074294.
- Biological activities of thymosin beta4 defined by active sites in short peptide sequences. 2010. PMID: 20179146.
